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Low-Cost Methods for Molecular Characterization of Mutant Plants : Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols.

Bibliographic Details
Main Author: Till, Bradley J.
Other Authors: Jankowicz-Cieslak, Joanna., Huynh, Owen A., Beshir, Mayada M., Laport, Robert G., Hofinger, Bernhard J.
Format: eBook
Language:English
Published: Cham : Springer International Publishing AG, 2015.
Edition:1st ed.
Subjects:
Electronic books.
Online Access:Click to View
Table of Contents:
  • Intro
  • Foreword
  • Acknowledgements
  • Contents
  • Chapter 1: Introduction
  • 1.1 Background
  • 1.2 Methods Used to Isolate Genomic DNA from Plant Tissues
  • 1.3 Methods for the Discovery and Characterization of Induced and Natural Nucleotide Variation in Plant Genomes
  • References
  • Chapter 2: Health and Safety Considerations
  • 2.1 Guidelines
  • 2.2 Preparation of a Home-Made Chemical Spill Kit
  • Reference
  • Chapter 3: Sample Collection and Storage
  • 3.1 Background
  • 3.2 Materials
  • 3.3 Methods
  • References
  • Chapter 4: Low-Cost DNA Extraction
  • 4.1 Materials
  • 4.2 Methods
  • 4.2.1 Preparation of Silica Powder DNA Binding Solution
  • 4.2.2 Low-Cost Extraction of Genomic DNA
  • 4.3 Alternative Buffers for DNA Extraction
  • Reference
  • Chapter 5: PCR Amplification for Low-Cost Mutation Discovery
  • 5.1 Materials
  • 5.2 Methods
  • References
  • Chapter 6: Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples
  • 6.1 Materials
  • 6.2 Methods
  • Reference
  • Chapter 7: Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes from Common Weedy Pl...
  • 7.1 Materials
  • 7.2 Methods
  • 7.2.1 Enzyme Extraction
  • 7.2.2 Concentration of Enzymes Using Amicon Ultra 10 kDa MWCO Centrifugal Filter Devices (for 0.5 ml Starting Volume
  • in 1.5-m...
  • 7.2.3 Test of Mismatch Cleavage Activity
  • Reference
  • Chapter 8: Example Data
  • 8.1 Quality of Genomic DNA Obtained by Silica Powder-Based DNA Extraction Method
  • 8.2 Quality of Genomic DNA Obtained by Silica Powder-Based DNA Extraction Method Using Alternative Buffers
  • 8.2.1 Summary
  • 8.3 Example of PCR Products Using TILLING Primers with Source Genomic DNA from a Commercial Kit and Low-Cost Silica Method
  • 8.4 Example of Low-Cost Agarose Gel-Based TILLING Assays for the Discovery of Induced Point Mutations.
  • 8.5 Example of Enzyme Activity Recovered from Weeds Compared to Crude Celery Juice Extract
  • 8.5.1 Summary
  • References
  • Chapter 9: Conclusions.

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