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03787nam a22004453i 4500 |
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MiAaPQ |
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20231204023215.0 |
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231204s2015 xx o ||||0 eng d |
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|a 9783319162591
|q (electronic bk.)
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|z 9783319162584
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|a (MiAaPQ)EBC6422689
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|a (Au-PeEL)EBL6422689
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|a (OCoLC)1231605307
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|a MiAaPQ
|b eng
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|c MiAaPQ
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|a TP248.27.P55
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|a Till, Bradley J.
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|a Low-Cost Methods for Molecular Characterization of Mutant Plants :
|b Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols.
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|a 1st ed.
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|a Cham :
|b Springer International Publishing AG,
|c 2015.
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|c ©2015.
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| 300 |
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|a 1 online resource (42 pages)
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| 336 |
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|a text
|b txt
|2 rdacontent
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|a computer
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|2 rdamedia
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|a online resource
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|2 rdacarrier
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|a Intro -- Foreword -- Acknowledgements -- Contents -- Chapter 1: Introduction -- 1.1 Background -- 1.2 Methods Used to Isolate Genomic DNA from Plant Tissues -- 1.3 Methods for the Discovery and Characterization of Induced and Natural Nucleotide Variation in Plant Genomes -- References -- Chapter 2: Health and Safety Considerations -- 2.1 Guidelines -- 2.2 Preparation of a Home-Made Chemical Spill Kit -- Reference -- Chapter 3: Sample Collection and Storage -- 3.1 Background -- 3.2 Materials -- 3.3 Methods -- References -- Chapter 4: Low-Cost DNA Extraction -- 4.1 Materials -- 4.2 Methods -- 4.2.1 Preparation of Silica Powder DNA Binding Solution -- 4.2.2 Low-Cost Extraction of Genomic DNA -- 4.3 Alternative Buffers for DNA Extraction -- Reference -- Chapter 5: PCR Amplification for Low-Cost Mutation Discovery -- 5.1 Materials -- 5.2 Methods -- References -- Chapter 6: Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples -- 6.1 Materials -- 6.2 Methods -- Reference -- Chapter 7: Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes from Common Weedy Pl... -- 7.1 Materials -- 7.2 Methods -- 7.2.1 Enzyme Extraction -- 7.2.2 Concentration of Enzymes Using Amicon Ultra 10 kDa MWCO Centrifugal Filter Devices (for 0.5 ml Starting Volume -- in 1.5-m... -- 7.2.3 Test of Mismatch Cleavage Activity -- Reference -- Chapter 8: Example Data -- 8.1 Quality of Genomic DNA Obtained by Silica Powder-Based DNA Extraction Method -- 8.2 Quality of Genomic DNA Obtained by Silica Powder-Based DNA Extraction Method Using Alternative Buffers -- 8.2.1 Summary -- 8.3 Example of PCR Products Using TILLING Primers with Source Genomic DNA from a Commercial Kit and Low-Cost Silica Method -- 8.4 Example of Low-Cost Agarose Gel-Based TILLING Assays for the Discovery of Induced Point Mutations.
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|a 8.5 Example of Enzyme Activity Recovered from Weeds Compared to Crude Celery Juice Extract -- 8.5.1 Summary -- References -- Chapter 9: Conclusions.
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|a Description based on publisher supplied metadata and other sources.
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|a Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, 2023. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries.
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| 655 |
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|a Electronic books.
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| 700 |
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|a Jankowicz-Cieslak, Joanna.
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| 700 |
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|a Huynh, Owen A.
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| 700 |
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|a Beshir, Mayada M.
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| 700 |
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|a Laport, Robert G.
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| 700 |
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|a Hofinger, Bernhard J.
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|i Print version:
|a Till, Bradley J.
|t Low-Cost Methods for Molecular Characterization of Mutant Plants
|d Cham : Springer International Publishing AG,c2015
|z 9783319162584
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| 797 |
2 |
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|a ProQuest (Firm)
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| 856 |
4 |
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|u https://ebookcentral.proquest.com/lib/matrademy/detail.action?docID=6422689
|z Click to View
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