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Alternatives to Animal Testing : Proceedings of Asian Congress 2016.

Bibliographic Details
Main Author: Kojima, Hajime.
Other Authors: Seidle, Troy., Spielmann, Horst.
Format: eBook
Language:English
Published: Singapore : Springer Singapore Pte. Limited, 2018.
Edition:1st ed.
Subjects:
Electronic books.
Online Access:Click to View
Table of Contents:
  • Intro
  • Contents
  • Zebrafish, Danio Rerio as a Replacement Alternative Model Useful in CKDu Experiments
  • Abstract
  • Introduction
  • The Concept of Replacement Alternative
  • Scientific Importance of Zebrafish (Danio Rerio)
  • Scientific Attributes of Zebrafish
  • Introduction of Zebrafish Model to Sri Lanka
  • Materials and Methods
  • Comprehensive Training on Zebrafish Model
  • Results
  • Discussion and Conclusion
  • Scientific Applicability of Zebrafish Model in the Sri Lankan Context
  • Acknowledgments
  • References
  • Testing Method Development and Validation for in Vitro Skin Irritation Testing (SIT) by Using Reconstructed Human Epidermis (RhE) Skin Equivalent - EPiTRI®
  • Abstract
  • Introduction
  • Materials and Methods
  • EPiTRI® Reconstructed Human Epidermis
  • Selection of Test Substances
  • Cell Viability Measurement by MTT Reduction
  • In Vitro Skin Irritation Test (SIT)
  • Prediction Model
  • Statistical Analysis
  • Results and Discussions
  • Change of TER and Structural Morphology during the Reconstruction of EPiTRI®
  • Quality Control of EPiTRI®
  • Protocol Refinements for the Skin Irritation Test of EPiTRI®
  • Modification of Washing Method for SIT
  • Chemical Exposure Time
  • Pre-incubation Time and Post-incubation Volume
  • Conclusion
  • References
  • Development the Technique for the Preparation and Characterization of Reconstructed Human Epidermis (RHE)
  • Abstract
  • Back Ground
  • Structure of Skin Networks
  • Epidermis
  • Dermis
  • Keratinocytes Cells
  • Melanocytes Cell
  • Fibroblasts Cell
  • Reconstruct Ed Human Epiderm is (Rhe)
  • Skin Irritation Test
  • Examination of Histology Network with Hematoxyline - Eosin Determination
  • Immunohistochemical (IHC)
  • Result and Discussion
  • Cell Culture of Keratinocytes, Melanocytes and Fibroblasts
  • Making of Feeder Layer (Dermis Eqivalen).
  • Making of RHE Tissue (Reconstructed Human Epidermis)
  • Measuring of Cell Life Using MTT Method
  • Making Histology Preparations of Reconstructed Human Epiderm Using Staining of Hematoxylin-Eosin (HE)
  • Immunohistochemical (IHC) Examination of RHE Skin Tissue Using Cytokeratin 10 Antibody Marker
  • Conclusion
  • References
  • Alternative Research (3Rs) in the World, Asia and Japan
  • The World
  • Asia
  • Japan
  • References
  • Approaches to Reducing Animal Use for Acute Toxicity Testing: Retrospective Analyses of Pesticide Data
  • Abstract
  • Introduction
  • Materials and Methods
  • Data Quality Evaluation
  • Categorization of Data
  • Analyses
  • Results
  • Distribution of Substances Among Hazard Categories
  • EPA Hazard Categories
  • GHS Hazard Categories
  • Concordance of Oral and Dermal Hazard Classifications
  • Discussion
  • Disclaimer
  • Acknowledgements
  • References
  • Progress in Eliminating One-Year Dog Studies for the Safety Assessment of Pesticides
  • Abstract
  • Introduction and Objective
  • Review of Regulatory Data not in the Public Domain
  • Results of the Studies Conducted in Germany
  • Results of the Studies Conducted by the US EPA
  • Relevance of the One-Year Dog Study in Assessing Human Health Risks for Registration of Pesticides. An Update to Include Pesticides Registered in Japan
  • Discussion
  • References
  • Cosmetic Regulation and Alternatives to Animal Experimentation in India
  • Abstract
  • Introduction
  • History of Ban of Animal Testing of Cosmetics in EU
  • Cosmetic Regulation in India
  • Ban on Use of Animals in Cosmetic Testing
  • Prohibition of Import of Cosmetics Tested on Animals
  • Conclusion
  • Acknowledgments
  • References
  • Guidance on the Use of Alternative Test Methods for the Safety Assessment of Cosmetics and Quasi-drugs
  • Abstract
  • MHLW Updates
  • JaCVAM Update.
  • Guidance for Alternative to Animal Testing
  • References
  • Alternatives and Refinement for Animal Experimentation in Cancer Research
  • Abstract
  • Introduction
  • Alternatives in Cancer Research
  • 3R's
  • CPCSEA
  • Refinement in Cancer Research
  • Conclusion
  • References
  • 3Rs in Quality Control of Human Vaccines: Opportunities and Barriers
  • Abstract
  • Animal Use in Vaccine Quality Control
  • 3Rs History and Vaccine Context
  • 3Rs Successes
  • Barriers to 3Rs
  • Perspectives for the Future
  • Concluding Remarks
  • References
  • The Use of Adverse Outcome Pathways (AOPs) to Support Chemical Safety Decisions Within the Context of Integrated Approaches to Testing and Assessment (IATA)
  • Abstract
  • Introduction
  • The Adverse Outcome Pathway (AOP) Framework
  • The Use of AOPs to Support Hazard and Risk Assessment
  • AOP-Supported IATA Case Study: Skin Sensitization
  • Summary
  • References
  • Mechanism-Based Evaluation System for Hepato- and Nephrotoxicity or Carcinogenicity Using Omics Technology
  • Abstract
  • Introduction
  • Chemicals, Animal Test and Microarray Analysis
  • AOP-Based Mechanism of Hepatotoxicity Suggested by Case Study with Carbon Tetrachloride
  • AOP-Based Mechanism of Nephrotoxicity Suggested by Case Study with Cisplatin
  • Detection System for Hepato- and Nephrotoxicity
  • Prediction System for Hepatic and Renal Carcinogenicity: CARCINOscreen®
  • Concluding Remarks
  • Acknowledgement
  • References
  • Alternative Methods for Developmental Toxicity Testing Using Mouse ESCs
  • Abstract
  • Introduction
  • Materials and Methods
  • Results
  • Discussion
  • Acknowledgement
  • References
  • Futuristic Approach to Alternative Model Organisms: Hydra Stakes Its Claim
  • Abstract
  • Introduction
  • Hydra-Systematics
  • Physiology and Anatomy
  • Reproduction
  • Regeneration
  • End Point Analysis.
  • Morphological Observation in Hydra Following Exposure to Chemicals
  • Methodology
  • Impact of Chemicals on Hydra Growth Rate
  • Methodology
  • Impact of Chemicals on Hydra's Regeneration Ability
  • Methodology
  • Impact of Chemicals on Hydra Cell Cycle
  • Methodology
  • Assessment of Cell Death in Hydra by ROS Generation and Apoptosis
  • Determination of ROS Generation in Hydra by H2-DCFDA Staining
  • Determination of Apoptosis by Acridine Orange and DAPI Staining
  • Acridine Orange Staining
  • 4′-6-Diamidino-2-Phenylindole (DAPI) Staining
  • Genotoxicity Assessment in Hydra Following Chemical Exposure
  • Methodology
  • Transcriptional Alterations in Hydra Following Chemicals Exposure
  • Conclusion
  • Acknowledgments
  • References
  • The Lush Prize and Young Researcher Asia Awards 2016
  • Abstract
  • Introduction
  • The Lush Prize Categories
  • The Black Box Prize
  • The Lush Prize Is a '1R' Initiative
  • Launch of Lush Prize 'Young Researcher Asia' Awards in 2016
  • Projects Funded by the Lush Prize to Date
  • References
  • Author Index.

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