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Pre-Field Screening Protocols for Heat-Tolerant Mutants in Rice.

Bibliographic Details
Main Author: Sarsu, Fatma.
Other Authors: Ghanim, Abdelbagi M. A., Das, Priyanka., Bahuguna, Rajeev N., Kusolwa, Paul Mbogo., Ashraf, Muhammed., Singla-Pareek, Sneh L., Pareek, Ashwani., Forster, Brian P., Ingelbrecht, Ivan.
Format: eBook
Language:English
Published: Cham : Springer International Publishing AG, 2018.
Edition:1st ed.
Subjects:
Electronic books.
Online Access:Click to View
Table of Contents:
  • Intro
  • Preface
  • Acknowledgements
  • Executive Summary
  • Contents
  • Chapter 1: General Introduction
  • 1.1 Background Analysis
  • 1.2 Physiology and Genetics of Heat Tolerance in Rice
  • 1.3 Physiological and Biochemical Heat Stress Indicators in Rice
  • 1.4 Breeding for Heat Tolerance in Rice
  • Chapter 2: Screening Protocols for Heat Tolerance in Rice at the Seedling and Reproductive Stages
  • 2.1 Background Analysis
  • 2.2 Screening Protocol for Heat Tolerance in Rice at the Seedling Stage
  • 2.2.1 Plant Materials
  • 2.2.2 Screening for Heat Tolerance Using Hydroponics
  • 2.2.2.1 Seedling Establishment in Hydroponics
  • 2.2.2.2 Care Required During Plant Growth in Hydroponics
  • 2.2.3 Screening for Heat Tolerance in Soil Using Pots
  • 2.2.4 Heat Treatment and Recovery of Seedlings
  • 2.2.5 Assessment of Heat Tolerance at the Seedling Stage
  • 2.2.6 Heat Tolerance Index (HTI)
  • 2.2.7 Recovery Stage After Heat Treatment
  • 2.3 Screening Protocol for Heat Tolerance in Rice at the Flowering Stage
  • 2.3.1 Germination and Seedling Establishment in Hydroponics and Pots
  • 2.3.2 Heat Treatment at the Flowering Stage
  • 2.3.3 Screening for Heat Tolerance at the Flowering Stage
  • Chapter 3: Validation of Screening Protocols for Heat Tolerance in Rice
  • 3.1 Background Analysis
  • 3.2 Validation of Physiological and Biochemical Indicators
  • 3.2.1 Physiological and Biochemical Characterization of Heat Tolerant Lines at the Seedling Stage
  • 3.2.2 Physiological and Biochemical Characterization of Heat Tolerant Lines at the Flowering Stage
  • 3.2.3 Methods
  • 3.2.3.1 Electrolyte Leakage Measurement
  • 3.2.3.2 Lipid Peroxidation Assay
  • 3.2.3.3 Measurement of Total Proteins
  • 3.2.3.4 Antioxidant Enzyme Activity Assay
  • 3.3 Validation Protocols of Rice Heat Tolerance Under Field Conditions
  • Chapter 4: Conclusion.
  • Correction to: Pre-Field Screening Protocols for Heat-Tolerant Mutants in Rice
  • References.

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