Table of Contents: The Impact of Food Bioactives on Health :

The Impact of Food Bioactives on Health : In Vitro and Ex Vivo Models.

Bibliographic Details
Main Author:	Verhoeckx, Kitty.
Other Authors:	Cotter, Paul., López-Expósito, Iván., Kleiveland, Charlotte., Lea, Tor., Mackie, Alan., Requena, Teresa., Swiatecka, Dominika., Wichers, Harry.
Format:	eBook
Language:	English
Published:	Cham : Springer International Publishing AG, 2015.
Edition:	1st ed.
Subjects:	Electronic books.
Online Access:	Click to View

Table of Contents:

Intro
Preface
General Introduction
Digestion and Absorption
Cells Present in the Intestine
Role of Microbiota
Contents
Part I: Gastrointestinal Digestion Models, General Introduction
General Introduction
References
Chapter 1: Static Digestion Models: General Introduction
1.1 Definition of Concepts: Bioavailability, Bioaccessibility and Bioactivity
1.2 Static Methods
1.2.1 Solubility/Dialyzability
1.2.2 Digestion Conditions
1.3 Applications: Advantages and Disadvantages
1.4 Static Versus In Vivo Digestion: Conclusions
References
Chapter 2: InfoGest Consensus Method
2.1 Introduction
2.2 The Oral Phase
2.3 The Gastric Phase
2.4 The Small Intestinal Phase
2.5 Practicalities
2.6 Sampling
2.7 Conclusions
References
Chapter 3: Approaches to Static Digestion Models
3.1 Introduction
3.2 Static Models for Protein Hydrolysis
3.3 Static Models for Lipid Hydrolysis
3.4 Other Static Models
References
Chapter 4: Dynamic Digestion Models: General Introduction
4.1 Geometry
4.2 Physical Forces
4.3 Biochemistry
References
Chapter 5: The TNO Gastro-Intestinal Model (TIM)
5.1 Introduction
5.2 Concept of TIM
5.3 TIM-1
5.4 TinyTIM
5.5 Advanced Gastric Compartment (TIM-agc)
5.6 The Use of TIM to Study the Bio-accessibility of Nutrients
5.7 Protein Quality
5.8 Prediction of Glycemic Response
5.9 Lipids
5.10 Conclusions
References
Chapter 6: Dynamic Gastric Model (DGM)
6.1 Origins and Design of the DGM
6.2 General Protocol for DGM Experiments
6.3 Uses of the DGM
6.3.1 Food-Based Research
6.3.2 Pharmaceutical-Based Research
6.4 Advantages, Disadvantages and Limitations
6.5 Availability of the System
References
Chapter 7: Human Gastric Simulator (Riddet Model)
7.1 Origins of the HGS.
7.2 Model Description
7.2.1 Gastric Compartment
7.2.2 Gastric Motility
7.2.3 Gastric Emptying
7.2.4 Gastric Secretions
7.2.5 Temperature Control
7.3 Analysis of HGS Biomechanical Relevance
7.4 Operating Protocol
7.4.1 Preparation of a Food Bolus
7.4.2 Gastric Processing
7.5 Uses of the HGS
7.5.1 Role of ACW Activity on Food Digestion
7.5.2 Role of Food Material Properties
7.6 Advantages and Limitations
7.7 Availability of the System
References
Chapter 8: The DIDGI® System
8.1 Origins and Design of the DIDGI® System
8.2 Validation of DIDGI® for the Digestion of Infant Formula
8.2.1 Protocol for the In Vitro Dynamic Digestion of Infant Formula Using the DIDGI® System
8.2.2 In Vivo Digestion of Infant Formula on Piglets
8.2.3 Comparison In Vitro/In Vivo Data
8.3 Advantages, Disadvantages and Limitations
8.4 Conclusion and Prospects
References
Part II: General Introduction to Cells, Cell Lines and Cell Culture
Introduction
Salt Solutions
Culture Media
Medium Quality
pH and CO2
Serum Addition
The Cell Culture Hood
Cell Culture Terminology (Fig. 1)
General Cell Culture Protocols
Trypsinisation and Subculturing of Cells
Passaging of Cells in Suspension Culture
Freezing Cells
The Thawing and Recovery of Cells
Cell Viability Testing
Contamination of Cell Cultures
References
Chapter 9: Epithelial Cell Models
General Introduction
9.1 Measurement of Transepithelial Electrical Resistance (TEER)
9.1.1 Basic Protocol
9.1.2 Calculating Transepithelial Resistance
9.2 Verification of Monolayer Integrity by Lucifer Yellow Flux
9.2.1 Basic Protocol
9.3 Summary
References
Chapter 10: Caco-2 Cell Line
10.1 Origin
10.2 Features and Mechanisms
10.3 Stability, Consistency and Reproducibility.
10.4 Relevance to Human In Vivo Situation
10.5 General Protocols for Caco-2 Cells
10.5.1 General Maintenance
10.5.2 Protocol for Polarizing Caco-2 Cells in Tissue Culture Inserts
10.5.3 Troubleshooting Guide for Transport Experiments Across Caco-2 Monolayers
10.6 Applications
10.7 Advantages and Disadvantages
10.8 Conclusion
References
Chapter 11: HT29 Cell Line
11.1 Origin
11.2 Features and Mechanisms
11.3 Stability, Consistency and Reproducibility
11.4 Relevance to Human In Vivo Situation
11.5 General Protocol for HT29-MTX Cells
11.5.1 Cell Maintenance Protocol
11.5.2 Experimental Protocol for Test Compounds
11.5.2.1 Study of the Mucin-Stimulating Activity
11.5.2.2 Evaluation of Transepithelial Absorption by Transwell® Inserts
11.6 Experimental Read Out
11.6.1 Functionality Studies
11.6.2 Transport Studies
11.6.3 Microorganisms Survival, Adhesion or Invasion
11.7 Conclusions
References
Chapter 12: The IPEC-J2 Cell Line
12.1 Origin
12.2 Special Features/Morphology/Receptors
12.3 Stability/Consistency/Reproducibility of the System
12.4 Relevance to Human In Vivo Situation
12.5 General Protocol
12.5.1 Culture Conditions
12.5.2 Experimental Readout
12.5.3 Sample Preparation
12.6 Conclusion
References
Chapter 13: Co-cultivation of Caco-2 and HT-29MTX
13.1 Origin, Features and Mechanisms
13.2 Stability/Consistency and Reproducibility
13.3 Relevance to the Human In Vivo Situation
13.4 General Protocol
13.5 Assess Viability
13.6 Experimental Readout
13.7 Advantages, Disadvantages and Limitations
13.8 Conclusions
References
Part III: Innate and Adaptive Immune Cells: General Introduction Iván López-Expósito
Monocytes and Macrophages
Dendritic Cells
Human Peripheral Blood Mononuclear Cells.
T Lymphocytes or T-Cells
References
Chapter 14: THP-1 and U937 Cells
14.1 Origin and Some Features of THP-1 and U937 Cells
14.2 Stability, Consistency and Reproducibility of the System
14.3 Relevance to Human In Vivo Situation
14.4 Other Models with the Same Applicability
14.5 General Protocol of Culturing THP-1 Cells
14.6 Differentiation of THP-1 and U937 Monocytes into Macrophages
14.7 Differentiation of THP-1 and U937 Monocytes into Dendritic Cells
14.8 Controls to Test Viability and Performance of the Model
14.9 Critical Notes
14.10 Read-Out of the System
References
Chapter 15: Peripheral Blood Mononuclear Cells
15.1 Origin
15.2 Features and Mechanisms
15.3 Stability, Consistency and Reproducibility
15.4 Relevance to Human In Vivo Situation
15.5 General Protocol
15.5.1 Study of Proliferative/Cytotoxic Activity
15.5.2 Study of Inflammatory Responses
15.6 Assess Viability
15.7 Experimental Read Out
15.8 Advantages, Disadvantages and Limitations of the System
15.9 Conclusions
References
Chapter 16: PBMC-Derived T Cells
16.1 Introduction and Origin
16.2 Features and Mechanisms
16.3 Applications of T Cell Cultures
16.4 General Protocol
16.4.1 T Cell Isolation Protocols
16.4.2 Indirect Positive Isolation of Human CD4+ T
16.4.2.1 Preparation of Cells and Antibodies
16.4.2.2 Coating of PBMCs with CD4 Antibody
16.4.2.3 Magnetic Beads Washing Procedure
16.4.2.4 Separation of T Cells
16.4.2.5 Detachment of T Cells from Beads
16.5 Assess Viability
16.6 Samples
16.7 Experimental Readouts
References
Chapter 17: Dendritic Cells
17.1 Origin
17.2 Features and Mechanisms
17.2.1 DC Subsets
17.2.1.1 Blood DCs
17.2.1.2 Mucosal DCs
17.2.1.3 Monocyte-Derived DCs
17.3 General Protocols
17.3.1 DC Cell Lines.
17.3.2 Isolating Primary DCs from Blood
17.3.3 CD34+-Derived DCs
17.3.4 Monocyte-Derived DCs
17.4 Asses Viability
17.5 Experimental Readout
17.5.1 Co-stimulation
17.5.2 Cytokine Production
17.5.3 Other DC Readouts
17.6 In Vitro Studies on Food Bioactives Using DCs (Table 17.3)
17.7 Critical Notes
References
Chapter 18: Co-culture Caco-2/Immune Cells
18.1 Origin, Features and Mechanisms
18.2 Relevance to Human In Vivo Situation
18.2.1 Co-culture Caco-2 and Dendritic Cells
18.2.2 Co-culture Caco-2 and B-cells (Raji)
18.3 Stability, Consistency and Reproducibility
18.4 General Protocol
18.4.1 Co-culture of Caco-2/Human Monocyte Derived DCs (Include Contact Dependent Events)
18.4.2 Caco-2/Human Monocyte Derived DCs (Soluble Factors)
18.4.3 Caco-2/THP-1 (Soluble Factors)
18.4.4 Caco-2/PBMCs (Soluble Factors)
18.4.5 Caco-2/B Cells
18.5 Assess Viability
18.6 Experimental Readout
18.7 Advantages, Disadvantages and Limitations
18.8 Conclusions
References
Part IV: Enteroendocrine Cell Models: General Introduction
References
Chapter 19: STC-1 Cells
19.1 Origin
19.2 Features and Mechanisms
19.3 Stability, Consistency and Reproducibility
19.4 Relevance to Human In Vivo Situation
19.5 General Protocol
19.5.1 Cell Maintenance Protocol
19.5.2 Experimental Protocol for Test Compounds
19.6 Assess Viability
19.7 Experimental Read out
19.8 Conclusions
References
Chapter 20: NCI-H716 Cells
20.1 Introduction
20.2 Origin
20.3 Features and Mechanisms
20.4 Stability/Consistency/Reproducibility
20.5 Relevance to the Human L-Cell In Vivo
20.6 General Protocol
20.6.1 Cell Maintenance Protocol
20.6.2 Experimental Protocol for Test Compounds
20.7 Assess Viability
20.8 Experimental Readout
20.9 Conclusions
References.
Chapter 21: Murine GLUTag Cells.

The Impact of Food Bioactives on Health : In Vitro and Ex Vivo Models.

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